Efficient genotyping of Schistosoma mansoni miracidia following whole genome amplification
نویسندگان
چکیده
منابع مشابه
Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia
BACKGROUND In areas where schistosomiasis control programs have been implemented, morbidity and prevalence have been greatly reduced. However, to sustain these reductions and move towards interruption of transmission, new tools for disease surveillance are needed. Genomic methods have the potential to help trace the sources of new infections, and allow us to monitor drug resistance. Large-scale...
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Whole genome amplification (WGA) technologies can be used to amplify genomic DNA when only small amounts of DNA are available. The Multiple Displacement Amplification Phi polymerase based amplification has been shown to accurately amplify DNA for a variety of genotyping assays; however, it has not been tested for genotyping many of the clinically relevant genes important for pharmacogenetic stu...
متن کاملA comparison of DNA pools constructed following whole genome amplification for two-stage SNP genotyping designs.
Genotyping in DNA pools reduces the cost and the time required to complete large genotyping projects. The aim of the present study was to evaluate pooling as part of a strategy for fine mapping in regions of significant linkage. Thirty-nine single nucleotide polymorphisms (SNPs) were analyzed in two genomic DNA pools of 384 individuals each and results compared with data after typing all indivi...
متن کاملSchistoDB: a Schistosoma mansoni genome resource
SchistoDB (http://schistoDB.net/) is a genomic database for the parasitic organism Schistosoma mansoni, one of the major causative agents of schistosomiasis worldwide. It currently incorporates sequences and annotation for S. mansoni in a single user-friendly database. Several genomic scale analyses are available as well as ESTs, oligonucleotides, metabolic pathways and drugs. In this article, ...
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ژورنال
عنوان ژورنال: Molecular and Biochemical Parasitology
سال: 2009
ISSN: 0166-6851
DOI: 10.1016/j.molbiopara.2009.02.010